Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Efficiency of XBP1 gene silencing in MCF7 and MDA-MB-231 cells. XBP1 expression was evaluated in MCF7 and MDA-MB-231 cells at the mRNA and protein levels by qPCR and western blot analysis, respectively. Protein expression was assessed following shRNA-2-mediated silencing. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of qPCR data was performed using one-way ANOVA, whereas western blot data were analyzed using Student’s t-test. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Western Blot, shRNA

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Representative microscopic images of transduced cells. Upper panels: MCF7; lower panels: MDA-MB-231. ( A ) Brightfield and ( B ) TurboGFP fluorescence images demonstrating transduction in the indicated cell lines. Images are representative of three independent experiments with similar results and were captured at 10X magnification, scale bars: 200 μm

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Fluorescence, Transduction

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Que-induced cytotoxicity and the combined effects of Que and XBP1 silencing on breast cancer cells viability. ( A , B ) Cell viability of MCF7 and MDA-MB-231 cells after treatment with increasing concentrations of Que (0–200 µM) for 24, 48, and 72 h. ( C ) Cell viability of MCF7 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (154.262 µM). ( D ) Cell viability of MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (192.103 µM). Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of cell viability was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Concentration Assay

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Colony formation capacity of MCF7 and MDA-MB-231 cells following Que treatment and XBP1 silencing. Representative colony images and quantitative colony counts are shown for MCF7 and MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups. Que was used at the IC 50 concentration determined for each cell line. Colonies were counted manually. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Concentration Assay

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Effects of Que, XBP1 silencing, and their combination on expression levels of apoptosis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of BAX, BCL2, CASP3, CASP7, CASP8, CASP9, CYCS, and FADD were analyzed using qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CASP3, CASP8, and CASP9 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Control, Western Blot, Concentration Assay

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Effects of Que, XBP1 silencing, and their combination on EMT- and metastasis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of EMT/metastasis-associated markers were analyzed by qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CDH1 and CDH2 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Control, Western Blot, Concentration Assay

Journal: 3 Biotech

Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

doi: 10.1007/s13205-026-04923-8

Figure Lengend Snippet: Proposed mechanistic overview of the combined treatment with Que and XBP1 silencing in MCF7 and MDA-MB-231 breast cancer cells. The schematic summarizes the treatment-associated reduction in cell viability, suppression of colony formation, modulation of apoptosis-associated markers, and EMT-related molecular changes observed in the present study. The relationships shown are presented as a conceptual summary of the current findings

Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: